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CartFeline Leukemia Inhibitory Factor (LIF) (Yeast-derived Recombinant Protein) - 25 micrograms
Bulk quantities of Feline LIF protein are available.
Please contact us for pricing.
Feline LIF (Leukemia Inhibitory Factor) Specifications
Molecular Weight (calculated) - 19.7kDa
Amino Acid Sequence - SPLPITPVNA TCATRHPCHS NLMNQIRNQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKTRL VELYRIIAYL GASLGNITRD QKVLNPNALS LHSKLNATAD IMRGLLSNVL CRLCNKYHVA HVDVAYGPDT SGKDVFQKKK LGCQLLGKYK QVIAVVAQAF (180)
Gene ID - 101090296
Homology Across Species
Felis catus (domestic cat) LIF – 100%
Acinonyx jubatus (cheetah) LIF – 100%
Callorhinus ursinus (northern fur seal) LIF – 100%
Eumetopias jubatus (Steller sea lion) LIF – 100%
Lynx canadensis (Canada lynx) LIF – 100%
Lynx pardinus (Spanish lynx) LIF – 100%
Odobenus rosmarus divergens (Pacific walrus) LIF – 100%
Panthera pardus (leopard) LIF – 100%
Panthera tigris altaica (Amur tiger) LIF – 100%
Puma concolor (puma) LIF – 100%
Zalophus californianus (California sea lion) LIF – 100%
Ailuropoda melanoleuca (giant panda) LIF – 99%
Leptonychotes weddellii (Weddell seal) LIF – 99%
Mustela putorius furo (domestic ferret) LIF – 99%
Ursus maritimus (polar bear) LIF – 98%
More - https://blast.ncbi.nlm.nih.gov/
Endotoxin - Naturally endotoxin-free
Applications
Cell Culture, ELISA Standard, Western Blot Control
LIF Background
Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.
Alternate Names - LIF, CDF, DIA, HILDA, MLPLI, leukemia inhibitory factor, interleukin 6 family cytokine, LIF interleukin 6 family cytokine
Endogenous pluripotent factor expression after reprogramming cat fetal fibroblasts using inducible transcription factors.
Zhou R, Comizzoli P, Keefer CL.
Mol Reprod Dev. 2019 Nov;86(11):1671-1681. doi: 10.1002/mrd.23257. Epub 2019 Aug 19.
Applications: Transduction of cat fetal fibroblasts
Inducing Pluripotency in the Domestic Cat (Felis catus).
Dutton LC, Dudhia J, Guest DJ, Connolly DJ.
Stem Cells Dev. 2019 Oct 1;28(19):1299-1309. doi: 10.1089/scd.2019.0142. Epub 2019 Sep 5.
Applications: Generation and culture of iPSCs
Abstract
Domestic cats suffer from a range of inherited genetic diseases, many of which display similarities with equivalent human conditions. Developing cellular models for these inherited diseases would enable drug discovery, benefiting feline health and welfare as well as enhancing the potential of cats as relevant animal models for translation to human medicine. Advances in our understanding of these diseases at the cellular level have come from the use of induced pluripotent stem cells (iPSCs). iPSCs can differentiate into virtually any cell type and can be derived from adult somatic cells, therefore overcoming the ethical implications of destroying embryos to obtain embryonic stem cells. No studies, however, report the generation of iPSCs from domestic cats [feline iPSCs (fiPSCs)]. Feline adipose-derived fibroblasts were infected with amphotropic retrovirus containing the coding sequences for human Oct4, Sox2, Klf4, cMyc, and Nanog. Isolated iPSC clones were expanded on inactivated mouse embryonic fibroblasts in the presence of feline leukemia inhibitory factor (fLIF). Retroviral delivery of human pluripotent genes gave rise to putative fiPSC colonies within 5-7 days. These iPS-like cells required fetal bovine serum and fLIF for maintenance. Colonies were domed with refractile edges, similar to mouse iPSCs. Immunocytochemistry demonstrated positive staining for stem cell markers: alkaline phosphatase, Oct4, Sox2, Nanog, and SSEA1. Cells were negative for SSEA4. Expression of endogenous feline Nanog was confirmed by quantitative polymerase chain reaction. The cells were able to differentiate in vitro into cells representative of the three germ layers. These results confirm the first generation of induced pluripotent stem cells from domestic cats. These cells will provide valuable models to study genetic diseases and explore novel therapeutic strategies.
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Kingfisher Biotech, Inc.
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Saint Paul, MN 55114
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